RT Journal
A1 Rosenberg, Marjorie
A1 Hui, Lester
A1 Ma, Junli
A1 Nusbaum, Harris C.
A1 Clark, Kevin
A1 Robinson, Louise
A1 Dziadzio, Laura
A1 Swain, Pamela M.
A1 Keith, Tim
A1 Hudson, Thomas J.
A1 Biesecker, Leslie G.
A1 Flint, Jonathan
T1 Characterization of Short Tandem Repeats from Thirty-One Human Telomeres
JF Genome Research 
JO Genome Research 
YR 1997 
FD September 01 
VO 7 
IS 9 
SP 917 
OP 923 
DO 10.1101/gr.7.9.917 
UL http://genome.cshlp.org/content/7/9/917.abstract 
AB Completion of genetic and physical maps requires markers from the ends (telomeres) of every human chromosome. We have searched for short tandem repeats (microsatellites) in cosmid and P1 clones and generated 661 sequence-tagged sites (STS) from the terminal 300 kb of 31 human chromosome ends. PCR assays were successfully designed for 58 microsatellites and mapped both genetically and on radiation hybrids (RHs) to confirm their telomeric location. Sequence analysis revealed marked variation in sequence composition, consistent with the hypothesis that even very highly GC-rich chromosome bands (the T bands) are not homogenous. The STSs that we have generated will be a necessary resource for the construction of physical maps of these complex regions of the genome.[Information about the microsatellites is available electronically at http://www.cshl.org/gr and sequence has been deposited in the Genome Database (GDB).]