RT Journal
A1 McPherson, John D.
A1 Apostol, Barbara
A1 Wagner-McPherson, Caryn B.
A1 Hakim, Simin
A1 Del Mastro, Richard G.
A1 Aziz, Naeema
A1 Baer, Elizabeth
A1 Gonzales, Genalyn
A1 Krane, Mary Carol
A1 Markovich, Rachelle
A1 Masny, Peter
A1 Ortega, Miguel
A1 Vu, John
A1 Vujicic, Marco
A1 Church, Deanna M.
A1 Segal, Allan
A1 Grady, Deborah L.
A1 Moyzis, Robert K.
A1 Spence, M. Anne
A1 Lovett, Michael
A1 Wasmuth, John J.
T1 A Radiation Hybrid Map of Human Chromosome 5 with Integration of Cytogenetic, Genetic, and Transcript Maps
JF Genome Research 
JO Genome Research 
YR 1997 
FD September 01 
VO 7 
IS 9 
SP 897 
OP 909 
DO 10.1101/gr.7.9.897 
UL http://genome.cshlp.org/content/7/9/897.abstract 
AB One of the major goals of the human genome project is to establish a physical map of each human chromosome with a density of sequence-tagged site (STS) markers exceeding one every 100 kb. We report here the generation of a human chromosome 5-specific radiation hybrid (RH) map that includes 556 markers. Of these markers, 132 loci are ordered with a maximum likelihood ratio of >1000:1 compared with the next most likely order. An additional 113 loci were ordered relative to these backbone markers with a maximum likelihood ratio of >10:1 but <1000:1. Together, these 245 loci form an ordered framework map for the chromosome. Using this framework, >300 more markers were localized based on two-point analysis with the ordered set. On average, there are 50 markers in common with the RH map presented here and other chromosome 5 maps included in the current whole genome cytogenetic, genetic, and physical maps. The accuracy of all the maps is evident in that there are no more than two discrepancies between any one of them and these data. All of the maps encompassing chromosome 5 complement each other providing excellent STS coverage with >2200 loci combined. The chromosome 5-specific RH map contains 20% of these independent loci. In addition, our RH map contains STSs derived from clones suitable for fluorescent in situ hybridization, allowing alignment to the cytogenetic map. Together, these maps will assist in the assembly of sequence-ready contigs and will aid in the identification of disease loci on chromosome 5 by positional cloning and positional candidate approaches.[The STS sequences described in this paper have been submitted to dbSTS under accession nos. G15666–G15715 andG16049–G16063. A complete map of human chromosome 5 is available as an on-line supplement at http://www.cshl.org/gr.]