RT Journal
A1 Greinwald, John H.
A1 Scott, Daryl A.
A1 Marietta, Jacquie R.
A1 Carmi, Rivka
A1 Manaligod, Jose
A1 Ramesh, Arabandi
A1 Zbar, Ross I.S.
A1 Kraft, Michelle L.
A1 Elbedour, Khalil
A1 Yairi, Yael
A1 Musy, Maurice
A1 Skvorak, Anne B.
A1 Van Camp, Guy
A1 Srisailapathy, C.R. Srikumari
A1 Lovett, Michael
A1 Morton, Cynthia C.
A1 Sheffield, Val C.
A1 Smith, Richard J.H.
T1 Construction of P1-Derived Artificial Chromosome and Yeast Artificial Chromosome Contigs Encompassing the DFNB7 andDFNB11 Region of Chromosome 9q13–21
JF Genome Research 
JO Genome Research 
YR 1997 
FD September 01 
VO 7 
IS 9 
SP 879 
OP 886 
DO 10.1101/gr.7.9.879 
UL http://genome.cshlp.org/content/7/9/879.abstract 
AB 
DFNB7 and DFNB11, two loci for autosomal recessive nonsyndromic hearing loss (ARNSHL), have been mapped to chromosome 9q13–21 in separate consanguineous families. Using a radiation hybrid map, we have determined the correct marker order in the DFNB7/11 region and have demonstrated that theDFNB11 locus resides within a redefined DFNB7interval. The gene(s) responsible for ARNSHL at these loci resides within an ∼1 cM interval bounded by markers D9S1806(centromeric) and D9S769 (telomeric). A recently discovered Indian family confirms the new telomeric boundary. To assist in the identification and cloning of candidate genes, YAC and PAC contigs were constructed. A total of 19 YAC and 23 PAC clones were utilized to span the affected region and ensure double coverage throughout. Twenty-two previously published STSs and 21 new STSs were used to determine marker order and confirm the integrity of the contig. Using a positional cloning strategy we have identified three cochlear expressed genes that map to the DFNB7/11 interval.