RT Journal
A1 Smith, Douglas R.
A1 Richterich, Peter
A1 Rubenfield, Marc
A1 Rice, Philip W.
A1 Butler, Carol
A1 Lee, Hong-Mei
A1 Kirst, Susan
A1 Gundersen, Kristin
A1 Abendschan, Kari
A1 Xu, Qinxue
A1 Chung, Maria
A1 Deloughery, Craig
A1 Aldredge, Tyler
A1 Maher, James
A1 Lundstrom, Ronald
A1 Tulig, Craig
A1 Falls, Kathleen
A1 Imrich, Joan
A1 Torrey, Dana
A1 Engelstein, Marcy
A1 Breton, Gary
A1 Madan, Deepika
A1 Nietupski, Raymond
A1 Seitz, Bruce
A1 Connelly, Steven
A1 McDougall, Steven
A1 Safer, Hershel
A1 Gibson, Rene
A1 Doucette-Stamm, Lynn
A1 Eiglmeier, Karin
A1 Bergh, Staffan
A1 Cole, Stewart T.
A1 Robison, Keith
A1 Richterich, Laura
A1 Johnson, Jason
A1 Church, George M.
A1 Mao, Jen-i
T1 Multiplex Sequencing of 1.5 Mb of the Mycobacterium leprae Genome
JF Genome Research 
JO Genome Research 
YR 1997 
FD August 01 
VO 7 
IS 8 
SP 802 
OP 819 
DO 10.1101/gr.7.8.802 
UL http://genome.cshlp.org/content/7/8/802.abstract 
AB The nucleotide sequence of 1.5 Mb of genomic DNA fromMycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to ∼66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 rRNAs, and 15 tRNAs. The gene density of one per 1.4 kb is slightly lower than that ofMycoplasma (1.2 kb). Of the protein coding genes, 44% have significant matches to genes with well-defined functions. Comparison of 1157 M. leprae and 1564 Mycobacterium tuberculosisproteins shows a complex mosaic of homologous genomic blocks with up to 22 adjacent proteins in conserved map order. Matches to known enzymatic, antigenic, membrane, cell wall, cell division, multidrug resistance, and virulence proteins suggest therapeutic and vaccine targets. Unusual features of the M. leprae genome include large polyketide synthase (pks) operons, inteins, and highly fragmented pseudogenes.[The sequence data described in this paper have been submitted to GenBank under accession nos. L78811–L78829,U00010–U00023, U15180–U15184, U15186, U15187, L01095, L01536, L04666, and L01263. On-line supplementary information for Table 1 is available at http://www.cshl.org/gr.]