TY  - JOUR
A1  - Smith, Douglas R.
A1  - Richterich, Peter
A1  - Rubenfield, Marc
A1  - Rice, Philip W.
A1  - Butler, Carol
A1  - Lee, Hong-Mei
A1  - Kirst, Susan
A1  - Gundersen, Kristin
A1  - Abendschan, Kari
A1  - Xu, Qinxue
A1  - Chung, Maria
A1  - Deloughery, Craig
A1  - Aldredge, Tyler
A1  - Maher, James
A1  - Lundstrom, Ronald
A1  - Tulig, Craig
A1  - Falls, Kathleen
A1  - Imrich, Joan
A1  - Torrey, Dana
A1  - Engelstein, Marcy
A1  - Breton, Gary
A1  - Madan, Deepika
A1  - Nietupski, Raymond
A1  - Seitz, Bruce
A1  - Connelly, Steven
A1  - McDougall, Steven
A1  - Safer, Hershel
A1  - Gibson, Rene
A1  - Doucette-Stamm, Lynn
A1  - Eiglmeier, Karin
A1  - Bergh, Staffan
A1  - Cole, Stewart T.
A1  - Robison, Keith
A1  - Richterich, Laura
A1  - Johnson, Jason
A1  - Church, George M.
A1  - Mao, Jen-i
T1  - Multiplex Sequencing of 1.5 Mb of the Mycobacterium leprae Genome
Y1  - 1997/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 802 
EP  - 819 
DO  - 10.1101/gr.7.8.802 
VL  - 7 
IS  - 8 
UR  - http://genome.cshlp.org/content/7/8/802.abstract 
N2  - The nucleotide sequence of 1.5 Mb of genomic DNA fromMycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to ∼66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 rRNAs, and 15 tRNAs. The gene density of one per 1.4 kb is slightly lower than that ofMycoplasma (1.2 kb). Of the protein coding genes, 44% have significant matches to genes with well-defined functions. Comparison of 1157 M. leprae and 1564 Mycobacterium tuberculosisproteins shows a complex mosaic of homologous genomic blocks with up to 22 adjacent proteins in conserved map order. Matches to known enzymatic, antigenic, membrane, cell wall, cell division, multidrug resistance, and virulence proteins suggest therapeutic and vaccine targets. Unusual features of the M. leprae genome include large polyketide synthase (pks) operons, inteins, and highly fragmented pseudogenes.[The sequence data described in this paper have been submitted to GenBank under accession nos. L78811–L78829,U00010–U00023, U15180–U15184, U15186, U15187, L01095, L01536, L04666, and L01263. On-line supplementary information for Table 1 is available at http://www.cshl.org/gr.] 
ER  -