@article{Smith01081997,
author = {Smith, Douglas R. and Richterich, Peter and Rubenfield, Marc and Rice, Philip W. and Butler, Carol and Lee, Hong-Mei and Kirst, Susan and Gundersen, Kristin and Abendschan, Kari and Xu, Qinxue and Chung, Maria and Deloughery, Craig and Aldredge, Tyler and Maher, James and Lundstrom, Ronald and Tulig, Craig and Falls, Kathleen and Imrich, Joan and Torrey, Dana and Engelstein, Marcy and Breton, Gary and Madan, Deepika and Nietupski, Raymond and Seitz, Bruce and Connelly, Steven and McDougall, Steven and Safer, Hershel and Gibson, Rene and Doucette-Stamm, Lynn and Eiglmeier, Karin and Bergh, Staffan and Cole, Stewart T. and Robison, Keith and Richterich, Laura and Johnson, Jason and Church, George M. and Mao, Jen-i}, 
title = {Multiplex Sequencing of 1.5 Mb of the Mycobacterium leprae Genome},
volume = {7}, 
number = {8}, 
pages = {802-819}, 
year = {1997}, 
doi = {10.1101/gr.7.8.802}, 
abstract ={The nucleotide sequence of 1.5 Mb of genomic DNA fromMycobacterium leprae was determined using computer-assisted multiplex sequencing technology. This brings the 2.8-Mb M. leprae genome sequence to ∼66% completion. The sequences, derived from 43 recombinant cosmids, contain 1046 putative protein-coding genes, 44 repetitive regions, 3 rRNAs, and 15 tRNAs. The gene density of one per 1.4 kb is slightly lower than that ofMycoplasma (1.2 kb). Of the protein coding genes, 44% have significant matches to genes with well-defined functions. Comparison of 1157 M. leprae and 1564 Mycobacterium tuberculosisproteins shows a complex mosaic of homologous genomic blocks with up to 22 adjacent proteins in conserved map order. Matches to known enzymatic, antigenic, membrane, cell wall, cell division, multidrug resistance, and virulence proteins suggest therapeutic and vaccine targets. Unusual features of the M. leprae genome include large polyketide synthase (pks) operons, inteins, and highly fragmented pseudogenes.[The sequence data described in this paper have been submitted to GenBank under accession nos. L78811–L78829,U00010–U00023, U15180–U15184, U15186, U15187, L01095, L01536, L04666, and L01263. On-line supplementary information for Table 1 is available at http://www.cshl.org/gr.]}, 
URL = {http://genome.cshlp.org/content/7/8/802.abstract}, 
eprint = {http://genome.cshlp.org/content/7/8/802.full.pdf+html}, 
journal = {Genome Research} 
}