TY - JOUR A1 - Heus, Joris J. A1 - de Winther, Menno P.J. A1 - van de Vosse, Esther A1 - van Ommen, Gert-Jan B. A1 - den Dunnen, Johan T. T1 - Centromeric and Noncentromeric ADE2-Selectable Fragmentation Vectors for Yeast Artificial Chromosomes in AB1380 Y1 - 1997/06/01 JF - Genome Research JO - Genome Research SP - 657 EP - 660 DO - 10.1101/gr.7.6.657 VL - 7 IS - 6 UR - http://genome.cshlp.org/content/7/6/657.abstract N2 - We have constructed a set of fragmentation vectors for the truncation of either the centromeric or the noncentromeric end of YACs containing a human DNA insert. These vectors carry ADE2 orHIS5 as the selectable marker, enabling direct use in AB1380, the host strain of most publicly available YAC libraries. Centromeric fragmentation vectors for AB1380 have not been reported previously; the noncentromeric vectors show high frequencies of fragmentation. ER -