RT Journal
A1 Stewart, Elizabeth A.
A1 McKusick, Kathleen B.
A1 Aggarwal, Amita
A1 Bajorek, Ewa
A1 Brady, Shannon
A1 Chu, Angela
A1 Fang, Nicole
A1 Hadley, David
A1 Harris, Mark
A1 Hussain, Sami
A1 Lee, Richard
A1 Maratukulam, Annu
A1 O’Connor, Kyle
A1 Perkins, Shanti
A1 Piercy, Mark
A1 Qin, Fawn
A1 Reif, Tim
A1 Sanders, Carla
A1 She, Xiaohong
A1 Sun, Wei-Lin
A1 Tabar, Poroshat
A1 Voyticky, Susan
A1 Cowles, Sid
A1 Fan, Jian-Bing
A1 Mader, Chris
A1 Quackenbush, John
A1 Myers, Richard M.
A1 Cox, David R.
T1 An STS-Based Radiation Hybrid Map of the Human Genome
JF Genome Research 
JO Genome Research 
YR 1997 
FD May 01 
VO 7 
IS 5 
SP 422 
OP 433 
DO 10.1101/gr.7.5.422 
UL http://genome.cshlp.org/content/7/5/422.abstract 
AB We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is <5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb.