TY - JOUR A1 - Stewart, Elizabeth A. A1 - McKusick, Kathleen B. A1 - Aggarwal, Amita A1 - Bajorek, Ewa A1 - Brady, Shannon A1 - Chu, Angela A1 - Fang, Nicole A1 - Hadley, David A1 - Harris, Mark A1 - Hussain, Sami A1 - Lee, Richard A1 - Maratukulam, Annu A1 - O’Connor, Kyle A1 - Perkins, Shanti A1 - Piercy, Mark A1 - Qin, Fawn A1 - Reif, Tim A1 - Sanders, Carla A1 - She, Xiaohong A1 - Sun, Wei-Lin A1 - Tabar, Poroshat A1 - Voyticky, Susan A1 - Cowles, Sid A1 - Fan, Jian-Bing A1 - Mader, Chris A1 - Quackenbush, John A1 - Myers, Richard M. A1 - Cox, David R. T1 - An STS-Based Radiation Hybrid Map of the Human Genome Y1 - 1997/05/01 JF - Genome Research JO - Genome Research SP - 422 EP - 433 DO - 10.1101/gr.7.5.422 VL - 7 IS - 5 UR - http://genome.cshlp.org/content/7/5/422.abstract N2 - We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markers. Comparison of the orders of markers on this map with orders derived from independent meiotic and YAC STS-content maps indicates that the error rate in defining high-confidence bins is <5%. Analysis of 322 random cDNAs indicates that the map covers the vast majority of the human genome. This STS-based radiation hybrid map of the human genome brings us one step closer to the goal of a physical map containing 30,000 unique ordered landmarks with an average marker spacing of 100 kb. ER -