TY  - JOUR
A1  - Haff, Lawrence A.
A1  - Smirnov, Igor P.
T1  - Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass Spectrometry
Y1  - 1997/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 378 
EP  - 388 
DO  - 10.1101/gr.7.4.378 
VL  - 7 
IS  - 4 
UR  - http://genome.cshlp.org/content/7/4/378.abstract 
N2  - We report a simple method, the PinPoint assay, for detecting and identifying single-base variations (polymorphisms) at specific locations within DNA sequences. An oligonucleotide primer is annealed to the target DNA immediately upstream of the polymorphic site and is extended by a single base in the presence of all four dideoxynucleotide triphosphates and a thermostable DNA polymerase. The extension products are desalted, concentrated, and subjected to delayed-extraction MALDI-TOF mass spectrometry. The base at the polymorphic site is identified by the mass added onto the primer. Heterozygous targets produce two mass-resolved species that represent the addition of both bases complementary to those at the polymorphic site. The assay is suitable for double-stranded PCR products without purification or strand separation. More than one primer can be simultaneously extended and then mass-analyzed. The mass spectrometric method thus shows promise for high-volume diagnostic or genotyping applications. 
ER  -