RT Journal
A1 Yu, Wei
A1 Andersson, Björn
A1 Worley, Kim C.
A1 Muzny, Donna M.
A1 Ding, Yan
A1 Liu, Wen
A1 Ricafrente, Jennifer Y.
A1 Wentland, Meredith A.
A1 Lennon, Greg
A1 Gibbs, Richard A.
T1 Large-Scale Concatenation cDNA Sequencing
JF Genome Research 
JO Genome Research 
YR 1997 
FD April 01 
VO 7 
IS 4 
SP 353 
OP 358 
DO 10.1101/gr.7.4.353 
UL http://genome.cshlp.org/content/7/4/353.abstract 
AB A total of 100 kb of DNA derived from 69 individual human brain cDNA clones of 0.7–2.0 kb were sequenced by concatenated cDNA sequencing (CCS), whereby multiple individual DNA fragments are sequenced simultaneously in a single shotgun library. The method yielded accurate sequences and a similar efficiency compared with other shotgun libraries constructed from single DNA fragments (>20 kb). Computer analyses were carried out on 65 cDNA clone sequences and their corresponding end sequences to examine both nucleic acid and amino acid sequence similarities in the databases. Thirty-seven clones revealed no DNA database matches, 12 clones generated exact matches (≥98% identity), and 16 clones generated nonexact matches (57%–97% identity) to either known human or other species genes. Of those 28 matched clones, 8 had corresponding end sequences that failed to identify similarities. In a protein similarity search, 27 clone sequences displayed significant matches, whereas only 20 of the end sequences had matches to known protein sequences. Our data indicate that full-length cDNA insert sequences provide significantly more nucleic acid and protein sequence similarity matches than expressed sequence tags (ESTs) for database searching.[All 65 cDNA clone sequences described in this paper have been submitted to the GenBank data library under accession nos. U79240–U79304.]