TY  - JOUR
A1  - Touchman, J W
A1  - Bouffard, G G
A1  - Weintraub, L A
A1  - Idol, J R
A1  - Wang, L
A1  - Robbins, C M
A1  - Nussbaum, J C
A1  - Lovett, M
A1  - Green, E D
T1  - 2006 expressed-sequence tags derived from human chromosome 7-enriched cDNA libraries.
Y1  - 1997/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 281 
EP  - 292 
DO  - 10.1101/gr.7.3.281 
VL  - 7 
IS  - 3 
UR  - http://genome.cshlp.org/content/7/3/281.abstract 
N2  - The establishment and mapping of gene-specific DNA sequences greatly complement the ongoing efforts to map and sequence all human chromosomes. To facilitate our studies of human chromosome 7, we have generated and analyzed 2006 expressed-sequence tags (ESTs) derived from a collection of direct selection cDNA libraries that are highly enriched for human chromosome 7 gene sequences. Similarity searches indicate that approximately two-thirds of the ESTs are not represented by sequences in the public databases, including those in dbEST. In addition, a large fraction (68%) of the ESTs do not have redundant or overlapping sequences within our collection. Human DNA-specific sequence-tagged sites (STSs) have been developed from 190 of the ESTs. Remarkably, 180 (96%) of these STSs map to chromosome 7, demonstrating the robustness of chromosome enrichment in constructing the direct selection cDNA libraries. Thus far, 140 of these EST-specific STSs have been assigned unequivocally to YAC contigs that are distributed across the chromosome. Together, these studies provide > 2000 ESTs highly enriched for chromosome 7 gene sequences, 180 new chromosome 7 STSs corresponding to ESTs, and a definitive demonstration of the ability to enrich for chromosome-specific cDNAs by direct selection. Furthermore, the libraries, sequence data, and mapping information will contribute to the construction of a chromosome 7 transcript map. 
ER  -