RT Journal
A1 Brady, Kevin P.
A1 Rowe, Lucy B.
A1 Her, Helen
A1 Stevens, Tamara J.
A1 Eppig, Janan
A1 Sussman, Daniel J.
A1 Sikela, James
A1 Beier, David R.
T1 Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis
JF Genome Research 
JO Genome Research 
YR 1997 
FD November 01 
VO 7 
IS 11 
SP 1085 
OP 1093 
DO 10.1101/gr.7.11.1085 
UL http://genome.cshlp.org/content/7/11/1085.abstract 
AB We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3′-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.