TY  - JOUR
A1  - Brady, Kevin P.
A1  - Rowe, Lucy B.
A1  - Her, Helen
A1  - Stevens, Tamara J.
A1  - Eppig, Janan
A1  - Sussman, Daniel J.
A1  - Sikela, James
A1  - Beier, David R.
T1  - Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis
Y1  - 1997/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1085 
EP  - 1093 
DO  - 10.1101/gr.7.11.1085 
VL  - 7 
IS  - 11 
UR  - http://genome.cshlp.org/content/7/11/1085.abstract 
N2  - We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3′-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism. 
ER  -