@article{Brady01111997, author = {Brady, Kevin P. and Rowe, Lucy B. and Her, Helen and Stevens, Tamara J. and Eppig, Janan and Sussman, Daniel J. and Sikela, James and Beier, David R.}, title = {Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis}, volume = {7}, number = {11}, pages = {1085-1093}, year = {1997}, doi = {10.1101/gr.7.11.1085}, abstract ={We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3′-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.}, URL = {http://genome.cshlp.org/content/7/11/1085.abstract}, eprint = {http://genome.cshlp.org/content/7/11/1085.full.pdf+html}, journal = {Genome Research} }