RT Journal
A1 Marra, Marco A.
A1 Kucaba, Tamara A.
A1 Dietrich, Nicole L.
A1 Green, Eric D.
A1 Brownstein, Buddy
A1 Wilson, Richard K.
A1 McDonald, Ken M.
A1 Hillier, LaDeana W.
A1 McPherson, John D.
A1 Waterston, Robert H.
T1 High Throughput Fingerprint Analysis of Large-Insert Clones
JF Genome Research 
JO Genome Research 
YR 1997 
FD November 01 
VO 7 
IS 11 
SP 1072 
OP 1084 
DO 10.1101/gr.7.11.1072 
UL http://genome.cshlp.org/content/7/11/1072.abstract 
AB As part of the Human Genome Project, the Washington University Genome Sequencing Center has commenced systematic sequencing of human chromsome 7. To organize and supply the effort, we have undertaken the construction of sequence-ready physical maps for defined chromosomal intervals. Map construction is a serial process composed of three main activities. First, candidate STS-positive large-insert PAC and BAC clones are identified. Next, these candidate clones are subjected to fingerprint analysis. Finally, the fingerprint data are used to assemble sequence-ready maps. The fingerprinting method we have devised is key to the success of the overall approach. We present here the details of the method and show that the fingerprints are of sufficient quality to permit the construction of megabase-size contigs in defined regions of the human genome. We anticipate that the high throughput and precision characteristic of our fingerprinting method will make it of general utility.