RT Journal
A1 Rhodes, M
A1 Dearlove, A
A1 Straw, R
A1 Fernando, S
A1 Evans, A
A1 Greener, M
A1 Lacey, T
A1 Kelly, M
A1 Gibson, K
A1 Brown, S D
A1 Mundy, C
T1 High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.
JF Genome Research 
JO Genome Research 
YR 1997 
FD January 01 
VO 7 
IS 1 
SP 81 
OP 86 
DO 10.1101/gr.7.1.81 
UL http://genome.cshlp.org/content/7/1/81.abstract 
AB The use of fluorescent end-labeled primers has proved successful for rapid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs can be prohibitive when a wide range of markers is to be analyzed for only a few genotypings. This particularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high-resolution genetic maps for positional cloning. We demonstrate here the use of fluorescent dUTPs for cost-effective, high-throughput microsatellite genotyping in the mouse. This alternative to the use of fluorescent end-labeled primers for semiautomated genotyping is potentially applicable to the construction of linkage maps in other species.