TY  - JOUR
A1  - Rhodes, M
A1  - Dearlove, A
A1  - Straw, R
A1  - Fernando, S
A1  - Evans, A
A1  - Greener, M
A1  - Lacey, T
A1  - Kelly, M
A1  - Gibson, K
A1  - Brown, S D
A1  - Mundy, C
T1  - High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.
Y1  - 1997/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 81 
EP  - 86 
DO  - 10.1101/gr.7.1.81 
VL  - 7 
IS  - 1 
UR  - http://genome.cshlp.org/content/7/1/81.abstract 
N2  - The use of fluorescent end-labeled primers has proved successful for rapid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs can be prohibitive when a wide range of markers is to be analyzed for only a few genotypings. This particularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high-resolution genetic maps for positional cloning. We demonstrate here the use of fluorescent dUTPs for cost-effective, high-throughput microsatellite genotyping in the mouse. This alternative to the use of fluorescent end-labeled primers for semiautomated genotyping is potentially applicable to the construction of linkage maps in other species. 
ER  -