TY - JOUR A1 - Sonstegard, T S A1 - Abel Ponce de León, F A1 - Beattie, C W A1 - Kappes, S M T1 - A chromosome-specific microdissected library increases marker density on bovine chromosome 1. Y1 - 1997/01/01 JF - Genome Research JO - Genome Research SP - 76 EP - 80 DO - 10.1101/gr.7.1.76 VL - 7 IS - 1 UR - http://genome.cshlp.org/content/7/1/76.abstract N2 - Genetic resolution of bovine chromosome 1 (BTA1) linkage group was significantly increased by screening for microsatellite clones a microdissected library constructed from a bovine cell line carrying a t(1;29) translocation. Eighty-five percent of the microsatellites (ms) (46/54) identified were informative in the USDA/MARC mapping population, and 96% of these ms (44/46) linked to BTA1 (LOD > 3.0). When merged with 40 existing BTA1 markers the genetic map spanned 153.8 cM (sex-averaged interval, 1.9 cM). The fourfold improvement in marker density of BTA1 provides a genetic map that enhances mapping of quantitative trait loci and implementation of marker assisted selection. ER -