TY - JOUR A1 - Ning, Y A1 - Roschke, A A1 - Christian, S L A1 - Lesser, J A1 - Sutcliffe, J S A1 - Ledbetter, D H T1 - Identification of a novel paternally expressed transcript adjacent to snRPN in the Prader-Willi syndrome critical region. Y1 - 1996/08/01 JF - Genome Research JO - Genome Research SP - 742 EP - 746 DO - 10.1101/gr.6.8.742 VL - 6 IS - 8 UR - http://genome.cshlp.org/content/6/8/742.abstract N2 - Small nuclear ribonucleoprotein-associated polypeptide N (snRPN) and an anonymous transcript, PAR-5, are two of the paternally expressed transcripts mapped to the Prader-Willi syndrome critical region. Using long-range PCR, we have isolated the genomic interval between snRPN and PAR-5, identified a novel transcript in this region, and termed it PAR-SN. Northern analysis demonstrates that PAR-SN is expressed in brain, skeletal muscle, and heart. Like snRPN and PAR-5, PAR-SN is expressed exclusively from the paternal homolog in cultured lymphoblasts. Sequence analysis of the transcript revealed no significant open reading frame but did include a polymorphic dinucleotide repeat (CA)17. ER -