RT Journal
A1 Inazuka, M
A1 Tahira, T
A1 Hayashi, K
T1 One-tube post-PCR fluorescent labeling of DNA fragments.
JF Genome Research 
JO Genome Research 
YR 1996 
FD June 01 
VO 6 
IS 6 
SP 551 
OP 557 
DO 10.1101/gr.6.6.551 
UL http://genome.cshlp.org/content/6/6/551.abstract 
AB A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis.