TY  - JOUR
A1  - Inazuka, M
A1  - Tahira, T
A1  - Hayashi, K
T1  - One-tube post-PCR fluorescent labeling of DNA fragments.
Y1  - 1996/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 551 
EP  - 557 
DO  - 10.1101/gr.6.6.551 
VL  - 6 
IS  - 6 
UR  - http://genome.cshlp.org/content/6/6/551.abstract 
N2  - A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis. 
ER  -