@article{Inazuka01061996,
author = {Inazuka, M and Tahira, T and Hayashi, K}, 
title = {One-tube post-PCR fluorescent labeling of DNA fragments.},
volume = {6}, 
number = {6}, 
pages = {551-557}, 
year = {1996}, 
doi = {10.1101/gr.6.6.551}, 
abstract ={A method for fluorescent postlabeling of PCR products has been developed. The method uses Klenow fragment of DNA polymerase I that exchanges the 3'-terminal residue of PCR-amplified DNA fragment for fluorescent nucleotides. All reactions, including PCR, are performed in one tube simply by successive addition of reagents. The products can be applied directly to fluorescence-based automated DNA sequencers without purification for either length determination in denaturing electrophoresis or mutation detection in SSCP electrophoresis.}, 
URL = {http://genome.cshlp.org/content/6/6/551.abstract}, 
eprint = {http://genome.cshlp.org/content/6/6/551.full.pdf+html}, 
journal = {Genome Research} 
}