RT Journal
A1 Sorokin, A
A1 Lapidus, A
A1 Capuano, V
A1 Galleron, N
A1 Pujic, P
A1 Ehrlich, S D
T1 A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.
JF Genome Research 
JO Genome Research 
YR 1996 
FD May 01 
VO 6 
IS 5 
SP 448 
OP 453 
DO 10.1101/gr.6.5.448 
UL http://genome.cshlp.org/content/6/5/448.abstract 
AB An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.