TY  - JOUR
A1  - Sorokin, A
A1  - Lapidus, A
A1  - Capuano, V
A1  - Galleron, N
A1  - Pujic, P
A1  - Ehrlich, S D
T1  - A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.
Y1  - 1996/05/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 448 
EP  - 453 
DO  - 10.1101/gr.6.5.448 
VL  - 6 
IS  - 5 
UR  - http://genome.cshlp.org/content/6/5/448.abstract 
N2  - An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes. 
ER  -