@article{Sorokin01051996,
author = {Sorokin, A and Lapidus, A and Capuano, V and Galleron, N and Pujic, P and Ehrlich, S D}, 
title = {A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing.},
volume = {6}, 
number = {5}, 
pages = {448-453}, 
year = {1996}, 
doi = {10.1101/gr.6.5.448}, 
abstract ={An efficient approach for structural studies on bacterial chromosomes is presented. It is based on high-resolution PCR map construction by using a multiplex long accurate PCR (MLA PCR) protocol and a YAC clone carrying the region to be mapped as indicator. The high-resolution PCR map of the bacillus subtilis rrnB-dnaB region is presented as an example. Data are also presented on the use of DNA generated by LA PCR for sequencing; they are relevant to LA PCR induced mutations and justify the application of such mapping for sequencing long stretches of bacterial chromosomes.}, 
URL = {http://genome.cshlp.org/content/6/5/448.abstract}, 
eprint = {http://genome.cshlp.org/content/6/5/448.full.pdf+html}, 
journal = {Genome Research} 
}