RT Journal
A1 Heid, C A
A1 Stevens, J
A1 Livak, K J
A1 Williams, P M
T1 Real time quantitative PCR.
JF Genome Research 
JO Genome Research 
YR 1996 
FD October 01 
VO 6 
IS 10 
SP 986 
OP 994 
DO 10.1101/gr.6.10.986 
UL http://genome.cshlp.org/content/6/10/986.abstract 
AB We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.