TY  - JOUR
A1  - Heid, C A
A1  - Stevens, J
A1  - Livak, K J
A1  - Williams, P M
T1  - Real time quantitative PCR.
Y1  - 1996/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 986 
EP  - 994 
DO  - 10.1101/gr.6.10.986 
VL  - 6 
IS  - 10 
UR  - http://genome.cshlp.org/content/6/10/986.abstract 
N2  - We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods. 
ER  -