@article{Heid01101996,
author = {Heid, C A and Stevens, J and Livak, K J and Williams, P M}, 
title = {Real time quantitative PCR.},
volume = {6}, 
number = {10}, 
pages = {986-994}, 
year = {1996}, 
doi = {10.1101/gr.6.10.986}, 
abstract ={We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.}, 
URL = {http://genome.cshlp.org/content/6/10/986.abstract}, 
eprint = {http://genome.cshlp.org/content/6/10/986.full.pdf+html}, 
journal = {Genome Research} 
}