@article{Watson01121995,
author = {Watson, J E and Slorach, E M and Maule, J and Lawson, D and Porteous, D J and Brookes, A J}, 
title = {Human repeat-mediated integration of selectable markers into somatic cell hybrids.},
volume = {5}, 
number = {5}, 
pages = {444-452}, 
year = {1995}, 
doi = {10.1101/gr.5.5.444}, 
abstract ={We describe a strategy to introduce preferentially the dominant selectable marker neoR into the human chromosome within a monochromosome hybrid cell line. Integration of a construct containing the marker is mediated by human-specific repeat elements that promote multilocus human-specific integration with a single targeting vector. We tested two classes of repeat elements: the Alu family of SINE repeats and the Line1 repeat family. We show that Alu sequences alone are insufficient to direct human-specific integration but when used in combination with a Line1 element, or when only Line1 elements are included, integration of the vector into the human component of a monochromosome somatic cell hybrid is favored. The vectors also carry sequences that facilitate mapping and selective cloning of the targeted region. This strategy provides a means to generate selectable human subchromosomal fragments that can be used for localization of genes through positional cloning and, more important, for the identification of functional units through DNA transfer.}, 
URL = {http://genome.cshlp.org/content/5/5/444.abstract}, 
eprint = {http://genome.cshlp.org/content/5/5/444.full.pdf+html}, 
journal = {Genome Research} 
}