RT Journal
A1 Byrappa, S
A1 Gavin, D K
A1 Gupta, K C
T1 A highly efficient procedure for site-specific mutagenesis of full-length plasmids using Vent DNA polymerase.
JF Genome Research 
JO Genome Research 
YR 1995 
FD November 01 
VO 5 
IS 4 
SP 404 
OP 407 
DO 10.1101/gr.5.4.404 
UL http://genome.cshlp.org/content/5/4/404.abstract 
AB Careful titration of Vent polymerase activity allows efficient amplification of full-length plasmids (12 kb). The high processivity and fidelity of this enzyme made oligonucleotide-directed site-specific mutagenesis of plasmids a straight-forward process. Using only two primers, a mutagenic and a complementary, single-base mutants of recombinant plasmids were obtained consistently with > 90% efficiency from a single round of PCR. This procedure also made site-specific deletion, insertion, and several bases mutagenesis facile and efficient.