RT Journal
A1 Shibata, H
A1 Tahira, T
A1 Hayashi, K
T1 RNA-primed PCR.
JF Genome Research 
JO Genome Research 
YR 1995 
FD November 01 
VO 5 
IS 4 
SP 400 
OP 403 
DO 10.1101/gr.5.4.400 
UL http://genome.cshlp.org/content/5/4/400.abstract 
AB We show that RNA can serve as a primer in PCR. Use of rTth DNA polymerase is essential because it has strong reverse transcriptase activity. RNA primers can be obtained by in vitro transcription and are less costly than DNA primers, which are chemically synthesized. RNA-primed PCR also opens the possibility that a specific amplification reaction can be achieved in the absence of knowledge of the target nucleotide sequence.