TY  - JOUR
A1  - Lamerdin, J E
A1  - Athwal, R S
A1  - Kansara, M S
A1  - Sandhu, A K
A1  - Patanjali, S R
A1  - Weissman, S M
A1  - Carrano, A V
T1  - Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.
Y1  - 1995/11/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 359 
EP  - 367 
DO  - 10.1101/gr.5.4.359 
VL  - 5 
IS  - 4 
UR  - http://genome.cshlp.org/content/5/4/359.abstract 
N2  - Expressed sequence tags (ESTs) from 298 clones have been generated from a randomly primed, normalized human adult thymus cDNA library. We describe the chromosomal localization of 136 of these ESTs by PCR-based mapping to a human monochromosomal somatic cell hybrid panel. Data base similarities to known genes are also described. A subset (n = 18) of these randomly primed ESTs extended the sequence of ESTs from other tissues currently in dbEST. Of the nonrepetitive human adult thymus ESTs generated in this study, 237 (79.5%) have no similarity to current data base entries. This would suggest that our collection contains approximately 100 new coding regions from thymus tissue, a large proportion of which likely will represent the middle regions of genes. The mapped ESTs should prove useful as new gene-based markers for mapping and candidate gene hunting, particularly when anchored to a well-developed physical map of the human genome. 
ER  -