@article{Lamerdin01111995, author = {Lamerdin, J E and Athwal, R S and Kansara, M S and Sandhu, A K and Patanjali, S R and Weissman, S M and Carrano, A V}, title = {Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.}, volume = {5}, number = {4}, pages = {359-367}, year = {1995}, doi = {10.1101/gr.5.4.359}, abstract ={Expressed sequence tags (ESTs) from 298 clones have been generated from a randomly primed, normalized human adult thymus cDNA library. We describe the chromosomal localization of 136 of these ESTs by PCR-based mapping to a human monochromosomal somatic cell hybrid panel. Data base similarities to known genes are also described. A subset (n = 18) of these randomly primed ESTs extended the sequence of ESTs from other tissues currently in dbEST. Of the nonrepetitive human adult thymus ESTs generated in this study, 237 (79.5%) have no similarity to current data base entries. This would suggest that our collection contains approximately 100 new coding regions from thymus tissue, a large proportion of which likely will represent the middle regions of genes. The mapped ESTs should prove useful as new gene-based markers for mapping and candidate gene hunting, particularly when anchored to a well-developed physical map of the human genome.}, URL = {http://genome.cshlp.org/content/5/4/359.abstract}, eprint = {http://genome.cshlp.org/content/5/4/359.full.pdf+html}, journal = {Genome Research} }