TY - JOUR A1 - Macina, R A A1 - Morii, K A1 - Hu, X L A1 - Negorev, D G A1 - Spais, C A1 - Ruthig, L A A1 - Riethman, H C T1 - Molecular cloning and RARE cleavage mapping of human 2p, 6q, 8q, 12q, and 18q telomeres. Y1 - 1995/10/01 JF - Genome Research JO - Genome Research SP - 225 EP - 232 DO - 10.1101/gr.5.3.225 VL - 5 IS - 3 UR - http://genome.cshlp.org/content/5/3/225.abstract N2 - Large terminal fragments of human chromosomes 2p, 6p, 8q, 12q, and 18q were cloned using yeast artificial chromosomes (YACs). RecA-assisted restriction endonuclease (RARE) cleavage analysis of genomic DNA samples from II unrelated individuals using YAC-derived probes confirmed the telomeric localizations of the half-YACs studied. The cloned fragments provide telomeric closure of maps for the respective chromosome arms and will supply the reagents needed for analyzing and sequencing these distal subtelomeric regions. ER -