RT Journal
A1 Del Mastro, R G
A1 Wang, L
A1 Simmons, A D
A1 Gallardo, T D
A1 Clines, G A
A1 Ashley, J A
A1 Hilliard, C J
A1 Wasmuth, J J
A1 McPherson, J D
A1 Lovett, M
T1 Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.
JF Genome Research 
JO Genome Research 
YR 1995 
FD September 01 
VO 5 
IS 2 
SP 185 
OP 194 
DO 10.1101/gr.5.2.185 
UL http://genome.cshlp.org/content/5/2/185.abstract 
AB To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.