TY - JOUR A1 - Del Mastro, R G A1 - Wang, L A1 - Simmons, A D A1 - Gallardo, T D A1 - Clines, G A A1 - Ashley, J A A1 - Hilliard, C J A1 - Wasmuth, J J A1 - McPherson, J D A1 - Lovett, M T1 - Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation. Y1 - 1995/09/01 JF - Genome Research JO - Genome Research SP - 185 EP - 194 DO - 10.1101/gr.5.2.185 VL - 5 IS - 2 UR - http://genome.cshlp.org/content/5/2/185.abstract N2 - To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation. ER -