@article{Del Mastro01091995, author = {Del Mastro, R G and Wang, L and Simmons, A D and Gallardo, T D and Clines, G A and Ashley, J A and Hilliard, C J and Wasmuth, J J and McPherson, J D and Lovett, M}, title = {Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.}, volume = {5}, number = {2}, pages = {185-194}, year = {1995}, doi = {10.1101/gr.5.2.185}, abstract ={To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.}, URL = {http://genome.cshlp.org/content/5/2/185.abstract}, eprint = {http://genome.cshlp.org/content/5/2/185.full.pdf+html}, journal = {Genome Research} }