RT Journal
A1 Krizman, D B
A1 Hofmann, T A
A1 DeSilva, U
A1 Green, E D
A1 Meltzer, P S
A1 Trent, J M
T1 Identification of 3'-terminal exons from yeast artificial chromosomes.
JF Genome Research 
JO Genome Research 
YR 1995 
FD June 01 
VO 4 
IS 6 
SP 322 
OP 326 
UL http://genome.cshlp.org/content/4/6/322.abstract 
AB We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences.