TY  - JOUR
A1  - Krizman, D B
A1  - Hofmann, T A
A1  - DeSilva, U
A1  - Green, E D
A1  - Meltzer, P S
A1  - Trent, J M
T1  - Identification of 3'-terminal exons from yeast artificial chromosomes.
Y1  - 1995/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 322 
EP  - 326 
VL  - 4 
IS  - 6 
UR  - http://genome.cshlp.org/content/4/6/322.abstract 
N2  - We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences. 
ER  -