@article{Krizman01061995,
author = {Krizman, D B and Hofmann, T A and DeSilva, U and Green, E D and Meltzer, P S and Trent, J M}, 
title = {Identification of 3'-terminal exons from yeast artificial chromosomes.},
volume = {4}, 
number = {6}, 
pages = {322-326}, 
year = {1995}, 
abstract ={We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences.}, 
URL = {http://genome.cshlp.org/content/4/6/322.abstract}, 
eprint = {http://genome.cshlp.org/content/4/6/322.full.pdf+html}, 
journal = {Genome Research} 
}