TY  - JOUR
A1  - Gardiner, D
A1  - Hartas, J
A1  - Currie, B
A1  - Mathews, J D
A1  - Kemp, D J
A1  - Sriprakash, K S
T1  - Vir typing: a long-PCR typing method for group A streptococci.
Y1  - 1995/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 288 
EP  - 293 
VL  - 4 
IS  - 5 
UR  - http://genome.cshlp.org/content/4/5/288.abstract 
N2  - We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This DNA was also used to develop a random amplified polymorphic DNA (RAPD) procedure. The discriminatory power of the two DNA-based procedures was compared with previous methods, M typing, and multilocus enzyme electrophoresis. Both procedures were highly discriminatory, but the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results. 
ER  -