@article{Gardiner01041995,
author = {Gardiner, D and Hartas, J and Currie, B and Mathews, J D and Kemp, D J and Sriprakash, K S}, 
title = {Vir typing: a long-PCR typing method for group A streptococci.},
volume = {4}, 
number = {5}, 
pages = {288-293}, 
year = {1995}, 
abstract ={We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This DNA was also used to develop a random amplified polymorphic DNA (RAPD) procedure. The discriminatory power of the two DNA-based procedures was compared with previous methods, M typing, and multilocus enzyme electrophoresis. Both procedures were highly discriminatory, but the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results.}, 
URL = {http://genome.cshlp.org/content/4/5/288.abstract}, 
eprint = {http://genome.cshlp.org/content/4/5/288.full.pdf+html}, 
journal = {Genome Research} 
}