TY  - JOUR
A1  - Lee, A
A1  - Kirk, J
A1  - Edmands, S
A1  - Radich, J
T1  - Multiplex PCR of bcr-abl fusion transcripts in Philadelphia positive acute lymphoblastic leukemia.
Y1  - 1995/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 283 
EP  - 287 
VL  - 4 
IS  - 5 
UR  - http://genome.cshlp.org/content/4/5/283.abstract 
N2  - We describe a multiplex PCR assay for the detection of bcr-abl fusion mRNA in Philadelphia chromosome positive acute lymphoblastic leukemia (Ph + ALL). The assay provides a quick method for screening p190 (e1:a2) and p210 (b2:a2 or b3:a2) bcr-abl mRNAs simultaneously. The assay proves to be highly sensitive with detection of as little as one positive bcr-abl-expressing cell in a background of 10(5) negative bcr-abl cells. Bone marrow and peripheral blood specimens from six patients were in total accordance when run by multiplex PCR and by the single primer PCR approach. The multiplex bcr-abl assay may prove to be highly useful for screening newly diagnosed patients with ALL for the bcr-abl fusion transcript and in following the course of disease during therapy. 
ER  -