TY - JOUR A1 - Quaresima, B A1 - Tiano, M T A1 - Porcellini, A A1 - D'Agostino, P A1 - Faniello, M C A1 - Bevilacqua, M A A1 - Cimino, F A1 - Costanzo, F T1 - PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes. Y1 - 1994/10/01 JF - Genome Research JO - Genome Research SP - 85 EP - 88 VL - 4 IS - 2 UR - http://genome.cshlp.org/content/4/2/85.abstract N2 - The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region. ER -