RT Journal
A1 Kato, Shingo
A1 Masuda, Sachiko
A1 Shibata, Arisa
A1 Itoh, Takashi
A1 Sakamoto, Mitsuo
A1 Shirasu, Ken
A1 Ohkuma, Moriya
T1 Whole-genome sequencing of diverse 351 cultured prokaryotes including as-yet-unsequenced type strains
JF Genome Research 
JO Genome Research 
YR 2026 
FD April 01 
VO 36 
IS 4 
SP 875 
OP 884 
DO 10.1101/gr.281026.125 
UL http://genome.cshlp.org/content/36/4/875.abstract 
AB Genome sequences provide fundamental information for both basic and applied life sciences. Whole-genome sequencing is now requested for describing novel prokaryotic species and designating their type strains, which serve as representative and well-characterized strains of the species. Indeed, the number of sequenced prokaryotic genomes has been rapidly increasing. However, a considerable number of isolated strains, particularly technically demanding strains such as strict anaerobes and slow growers, remain without genome sequence information. Here we report the whole-genome sequencing of 290 bacterial and 61 archaeal strains, including technically demanding type strains, obtained from the Japan Collection of Microorganisms (JCM) using a combination of short- and long-read sequencing technologies. The data set includes 284 type-strain genomes and 235 complete genomes. Notably, in the data set, genomes of more than 200 strains, including more than 150 type strains, have not been made publicly available. Comparative genomic analysis suggests that some strains need to be assigned to novel taxa or reclassified. Functional gene survey indicates that some strains possess previously unrecognized potential for carbon fixation or bioactive secondary metabolite production. Our data set will contribute to more accurate taxonomic classification, fill gaps in the phylogeny of prokaryotes, and provide insights into their physiology and ecology.