TY  - JOUR
A1  - Shemirani, Ruhollah
A1  - Belbin, Gillian M.
A1  - Cullina, Sinead
A1  - Caggiano, Christa
A1  - Gignoux, Christopher R.
A1  - Zaitlen, Noah
A1  - Kenny, Eimear E.
T1  - A spectral component approach leveraging identity-by-descent graphs to address recent population structure in genomic analysis
Y1  - 2026/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 534 
EP  - 546 
DO  - 10.1101/gr.280659.125 
VL  - 36 
IS  - 3 
UR  - http://genome.cshlp.org/content/36/3/534.abstract 
N2  - Population structure is a well-known confounder in statistical genetics, particularly in genome-wide association studies (GWAS), in which it can lead to inflated test statistics and spurious associations. Traditional methods, such as principal components (PCs), commonly used to adjust for population structure, are limited in capturing fine-scale, nonlinear patterns that arise from recent demographic events, patterns that are crucial for understanding rare variant effects. To address this challenge, we propose a novel method called spectral components (SPCs), which leverages identity-by-descent (IBD) graphs to capture and transform local, nonlinear fine-scale population structure into continuous representations that can be seamlessly integrated into genetic analysis pipelines. Using both simulated data sets and empirical data from the UK Biobank (N ≈ 420,000), we demonstrate that SPCs outperform PCs in adjusting for fine-scale population structure. In simulations, SPCs explain >90% of the fine-scale population structure with fewer components, whereas PCs capture <5%. In the UK Biobank, SPCs reduce the inflation of P-values in the GWAS of an environmental-driven phenotype by 12% compared with PCs, while maintaining a similar performance to PCs in height, a highly heritable phenotype. Additionally, SPCs improve rare variant association analyses, reducing genomic inflation (e.g., from 7.6 to 1.2 in one analysis), and provide more accurate heritability estimates. Spatial autocorrelation analysis further confirms the ability of SPCs to account for environmental effects, reducing Moran's I for both environmental and heritable phenotypes more effectively than PCs. Overall, our findings demonstrate that SPCs provide a robust, scalable adjustment for recent population structure, offering a powerful alternative or complement to PCs in large-scale biobank studies. 
ER  -