RT Journal
A1 Babik, Wiesław
A1 Dudek, Katarzyna
A1 Palomar, Gemma
A1 Marszałek, Marzena
A1 Dubin, Grzegorz
A1 Yun, Maximina H.
A1 Migalska, Magdalena
T1 MHC in newts illuminates the evolutionary dynamics of complex regions in giant genomes
JF Genome Research 
JO Genome Research 
YR 2026 
FD February 01 
VO 36 
IS 2 
SP 303 
OP 317 
DO 10.1101/gr.281127.125 
UL http://genome.cshlp.org/content/36/2/303.abstract 
AB Major Histocompatibility Complex (MHC) molecules are central to vertebrate adaptive immunity, and MHC genes serve as key models in evolutionary genomics, offering insight into birth-and-death evolution, gene duplication, and the maintenance of genetic diversity. However, the organization and evolution of the MHC in species with giant genomes, such as salamanders, remain poorly understood. Here, we use comparative genomics, expression across multiple ontogenetic stages and tissues, as well as polymorphism data to investigate MHC evolution in newts. Contrary to earlier suggestions of a massively expanded MHC in salamanders, we find that the core MHC region remains relatively compact, demonstrating that genome gigantism does not scale proportionally in this region. Our finding also challenges the model of coevolution between a single classical MHC-Ia gene and antigen processing genes (APGs), revealing instead several polymorphic and highly expressed putative MHC-Ia located at varying distances from the APGs. MHC-I genes exhibit lineage-specific duplications and signs of concerted evolution, resulting in poorly resolved phylogenies. In contrast, MHC-II genes are more conserved and exhibit extensive trans-species polymorphism. Expression and polymorphism patterns identify putative nonclassical MHC-Ib genes, likely repeatedly derived from MHC-Ia genes, paralleling patterns seen in mammals but contrasting with the situation in fish and Xenopus frogs. In all seven studied species, some MHC-Ib genes show high relative expression during the larval stage but not at adulthood, suggesting a role in larval immunity. Our results underscore the importance of salamanders for understanding the evolution of complex regions in giant genomes and the architecture of the tetrapod MHC.