RT Journal
A1 Mendoza-Ferri, Maria Grazia
A1 Lozachmeur, Gwendoline
A1 Duvina, Maximilien
A1 Perret, Laetitia
A1 Merciris, Didier
A1 Gigout, Anne
A1 Mendoza-Parra, Marco Antonio
T1 Tissular chromatin-state cartography based on double-barcoded DNA arrays that capture unloaded PA-Tn5 transposase
JF Genome Research 
JO Genome Research 
YR 2025 
FD July 01 
VO 35 
IS 7 
SP 1633 
OP 1645 
DO 10.1101/gr.280305.124 
UL http://genome.cshlp.org/content/35/7/1633.abstract 
AB Recent developments in spatial omics are revolutionizing our understanding of tissue structure organization and their deregulation in disease. Here, we present a strategy for capturing chromatin histone modification signatures across tissue sections by taking advantage of a double-barcoded DNA arrays design compatible with in situ Protein A–transposase Tn5 tagmentation. This approach has been validated in presence of fresh-frozen mouse brain tissues but also in decalcified formalin-fixed paraffin-embedded (FFPE) mouse paw samples, in which either the histone modification H3K4 trimethylation or H3K27 acetylation has been used as proxy for interrogating active promoter signatures. Furthermore, because combinatorial enrichment of multiple histone modifications was shown to code for various states of gene transcriptional status (active, bivalent, repressed), we have integrated several histone modifications generated from consecutive mouse embryo sections to reveal changes in chromatin states across the tissue. Overall, this spatial epigenomic technology combined with the use of a spatial chromatin-state analytical strategy paves the way for future epigenetics studies for addressing tissue architecture complexity.