TY  - JOUR
A1  - Baquero Pérez, Manuela
A1  - Laenen, Gertjan
A1  - Loïodice, Isabelle
A1  - Garnier, Mickaël
A1  - Szachnowski, Ugo
A1  - Morillon, Antonin
A1  - Ruault, Myriam
A1  - Taddei, Angela
T1  - Intergenic accumulation of RNA polymerase II maintains the potential for swift transcriptional restart upon release from quiescence
Y1  - 2025/10/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2226 
EP  - 2239 
DO  - 10.1101/gr.279874.124 
VL  - 35 
IS  - 10 
UR  - http://genome.cshlp.org/content/35/10/2226.abstract 
N2  - Quiescent (Q) cells are seemingly inactive, developmentally arrested cells, whose universal characteristic is the ability to promptly re-enter the cell cycle upon sensing of external cues. Q cells are responsive to the environment and flexible enough to adapt to available resources. In budding yeast, quiescent nuclear features are drastically distinct from those observed in nutrient replete conditions: The nuclear volume is reduced; the telomeres relocate from the nuclear periphery to the center of the nucleus into a hypercluster; chromatin is found in a compacted, hypoacetylated state; and transcription is globally shutdown. Yet, Q cells can restart transcription within minutes of refeeding. Here, we follow the global decrease of transcription in sorted, developing Q populations and its reactivation upon release. We find that transcription and telomere clustering dynamics in and out of quiescence are independent events. We report a genome-wide redistribution of the transcription machinery as cells progress into quiescence. Although most genes are shut down, 3% of coding genes remain active. Furthermore, RNA polymerase II (RNAPII) accumulates at one-third of gene promoters. The corresponding genes are highly enriched among those showing a high level of transcription and high frequency of expression in individual cells, shortly after cells are refed, as monitored by single-cell RNA-seq. Our results point toward a role for quiescent-specific RNAPII distribution to ensure a rapid and robust transcriptional response upon return to growth. 
ER  -